University of Missouri School of Medicine MU Health School of Medicine

Gerald A. Meininger, PhD

Gerald A. Meininger, PhD

Professor and Director
Dalton Cardiovascular Research Center

MeiningerG@missouri.edu

Research Interests

Research in this laboratory is focused on vascular physiology and cell biology with an emphasis the microvasculature.

Research Description

Research interests Meininger's laboratory have been focused on vascular physiology and cell biology with an emphasis the microvasculature. Currently active areas of research include:

  1. Cell adhesion, extracellular matrix and cell signaling in the vascular wall
  2. Mechanotransduction in vascular cells
  3. Regulation of vascular and cardiac responses to tissue injury by extracellular matrix derived signals
  4. Cellular and molecular mechanisms responsible for mechanotransduction
  5. Mechanisms of vascular remodeling
  6. Mechanisms responsible for the myogenic properties of vascular smooth muscle
  7. Application of fluorescence microscopy and 3D-image analysis for studies of microvascular cell biology and the cyto-architecture and function of the microvessel wall
  8. Mechanisms of blood flow autoregulation.

Laboratory models include study of the intact microcirculation, study of isolated arterioles, freshly dispersed or cultured vascular smooth muscle cells, endothelial cells and cardiac muscle cells. Examples of technical approaches include pharmacology of the intact microvasculature and isolated microvessels; ability to manipulate pressure and flow in isolated microvessels; vessel culture and transfection; immuno-cytochemistry of the microvessel wall and isolated cells; three-dimensional fluorescence imaging using confocal, multiphoton or wide field microscopy in combination with deconvolution; atomic force microscopy combined with fluorescence microscopy (TIRF and FRET); and software development for high through-put analysis and display of atomic force microscopy force data.

An emphasis over the last several years has been to understand the role of the extracellular matrix, adhesion molecules and the cytoskeleton in regulation of vascular and cardiac cells; especially in the control of contractile function. A fundamental aim of this work has been to determine to what extent this matrix-adhesion-cytoskeletal axis may be involved in mechanotransduction phenomena that underlie the vascular myogenic response, flow-dependent responses of the endothelium and vascular remodeling. Advances in hybridizing atomic force microscopy with fluorescence microscopy are permitting higher throughput evaluation of cell surface receptors and their interactions with specific ligands.

Professional Background

  • Obtained BS in biology, Central Michigan University.
  • Obtained MS in environmental physiology, Central Michigan University.
  • Obtained PhD in physiology, University of Missouri–Columbia.
  • Completed postdoctoral training, Texas A&M University Health Science Center
  • Academic Career from Assistant to Full Professor, Texas A&M University Health Sceince Center
  • Served as Regents Professor, associate department head, Department of Medical Physiology and director, Division of Vascular Biology, Cardiovascular Research Institute, Texas A&M University Health Science Center.
  • Joined University of Missouri-Columbia in 2005 as Director of Dalton Cardiovascular Research Center.
  • Received Established Investigator Award from the American Heart Association (AHA), 1988.

Extramural Grant Review

American Heart Association, National
  • Co-Chair, Cardiovascular Regulation Review Committee II, American Heart Association - National, 2000-2001.
  • Member, Cardiovascular Regulation Review Committee II, American Heart Association - National, 1996-2001.
  • Member, Cardiovascular Regulation Review Committee I, American Heart Association - National, 2001-2004.
  • Member, Vascular Wall Biology 2 Review Committee, American Heart Association - National, April and October, 2004-2005.
  • Co-Chair, Vascular Wall Biology 2 Review Committee, American Heart Association - National, April and October, 2006.
  • Chair, Vascular Wall Biology 2 Review Committee, American Heart Association - National, April and October, 2007, 2008.
American Heart Association, Western Review Consortium
  • Chair for Study Group A of the Central Research Review Committee for the American Heart Association, Texas Affiliate, November 1993-1994.
  • Vice Chair for Study Group A of the Central Research Review Committee for the American Heart Association, Texas Affiliate, November 1991-1993.
  • Member Research Allocations and Advisory Committee for the American Heart Association, Texas Affiliate, 1991-1995
  • Member Central Research Review Committee for the American Heart Association, Texas Affiliate, 1989-1995.
National Institutes of Health
  • Grant reviewer for Research Resource Center Grant (P41) Special emphasis panel/scientific review group for the National Center for Research Resources (NCRR), National Institutes of Health, December 2009.
  • Grant reviewer for NHLBI Proteomic Centers for heart, lung and blood diseases. National Institutes of Health, September 2009.
  • Grant reviewer for Research Resource Center Grant (P41) Site visit review team for the National Center for Research Resources (NCRR), National Institutes of Health, March 2009.
  • Grant reviewer for Shared Instrumentation Program (S10) Study Section panel of the National Center for Research Resources (NCRR), National Institutes of Health, October 2008.
  • Grant reviewer (ad hoc) for Modeling and Analysis of Biological Systems (MABS) Study Section at the Center for Scientific Review, National Institutes of Health, January 2008.
  • Member of Special Emphasis Panel for review of R13 Conference Grant Applications, Center for Scientific Review, National Institutes of Health, March 2007.
  • Member of Shared Instrumentation Microscopy (S10) Study Section panel, Center for Scientific Review, National Institutes of Health, September 2004-2006.
  • Member of Reparative Medicine -Tissue Engineering (MOSS G 01) Study Section panel, Center for Scientific Review, National Institutes of Health, July 2004-2006.
  • Temporary member of Reparative Medicine -Tissue Engineering Study Section (SSS-M), Center for Scientific Review, National Institutes of Health, June 2003.
  • Temporary member of Experimental Cardiovascular Sciences Study Section, Division of Research Grants, National Institutes of Health, March 1996-2004.
  • Program Project Grant review team for National Institutes of Health, Heart, Lung and Blood Institute. Cardiovascular and Renal Study Section, May 1992; April 1998; December 2000; October 2005; April 2006.
  • Grant reviewer (ad hoc) for National Institutes of Health, Experimental Cardiovascular Sciences Study Section, June 1993.
  • Grant reviewer (ad hoc) for National Institutes of Health, Metabolism Study Section, February 1993.
National Science Foundation
  • Grant reviewer (ad hoc) for the National Science Foundation, Washington D.C., Physiological Processes Program, 1991, 1998.
Veterans Administration
  • Proposal review of Merit Award for Research and Development Committee of Miami VA Medical Center, May 1992.
NASA
  • Grant reviewer (ad hoc) for NASA's Biomedical Research Program through the American Institute of Biological Sciences, October 1988.
Biotechnology and Biological Sciences Research Council (BBSRC)
  • Grant reviewer for United Kingdom, BBSRC's Technology Development Initiative Committee, March 2006.
Canada Foundation for Innovation
  • Grant review for Innovation fund of Canada Foundation for Innovation, July 2001.
Canadian Institutes of Health Research (CIHR)
  • Grant reviewer (ad hoc) for CIHR, July 2008.
Thomas F. & Kate Miller Jeffress Memorial Trust
  • Grant reviewer for a Jeffress Research Grant through the Thomas F. & Kate Miller Jeffress Memorial Trust, administered by Sovran Bank, N. A., Virginia Academy of Science, and the Medical Society of Virginia, March 1989; September 1994.
Croucher Foundation
  • Proposal reviewer for a Croucher Foundation Award, February 2008.

National Committees

American Heart Association
  • Abstract Reviewer and Grader, 1994-95, 1998-99, 2001-04.
  • Member American Heart Association Steering Committee, Unified Peer Review Panel representing the Midwest Programs, July 2009-June 2011.
American Physiological SocietyK
  • APS: Conference Committee, Chair, 2011-2013.
  • APS: SAC/Nominating Committee: CVS Representative, 2003-2004; 2007-2010.
  • APS: Strategic Planning Meeting, December 2005.
  • APS: Committee on Committees: Member, 2001-2003.
  • APS: AJP (Heart Circulatory Physiology) Associate Editor 1993-1999; 2007-2010.
Section Activity
  • Cardiovascular Section: Past Chair: 2010-2013.
  • Cardiovascular Section: Chair: 2007-2010.
  • Cardiovascular Section: Secretary/Treasurer, 2003-2006; 2006-2007 (elected Chair 2007).
  • Cardiovascular Section: Liaison Committee: Ad Hoc Member, 2004-2007.
  • Cardiovascular Section: Program Committee, 1999 to 2000.
  • Cardiovascular Section: Steering Committee: Member, 1999 to 2013.
  • Cardiovascular Section: Nominating Committee: Member, 1998-1999; Chair, 1999-2001.
  • Cardiovascular Section: Banquet Committee: Chair, 2010-2013.
European Society for Microcirculation
  • Ad hoc Publication Award Committee, 2000 – 2005.
International Union of Physiological Sciences
  • Ad hoc Publication Award Committee, 2000 – 2005.
  • Member, US National Committee to the International Union of Physiological Sciences, 2006-present.
  • Chair, Microcirculation Section of the IUPS Commission II (Circulation/Respiration), 2007-2009.
Microcirculatory Society
  • Past-President, 1996-1997.
  • President, 1995-1996.
  • President-Elect, 1994-1995.
  • Long Range Planning Committee: Chairman, 1996-1997, Member 1997-2007.
  • Liaison Committee: Chairman, 1995-1996. Member, 2006-2009, 2010-2013.
  • Development Committee: Member, 1989-1991; Chairman, 1991-1994
  • Executive Council: Member, 1990-1993.
  • Nominating Committee: Member, 1986-1987; Chairman, 1987-1989.

External Consulting

  • Invited external examiner and graduate student committee member for Dr. John Imig student of Professor Gary Anderson, Department of Physiology and Biophysics, School of Medicine, University of Louisville, KY, 1988-1990.
  • Invited external examiner and graduate student committee member for Dr. Pei-Pei Ping student of Professor Paul Johnson, Department of Physiology, College of Medicine, University of Arizona, 1990.
  • Invited external examiner and graduate student committee member for Yanlin Wang, Department of Internal Medicine, Cardiovascular Division, University of Texas Medical Branch at Galveston, 1997-1999.
  • External Review Team Member for Academic Program Review of the Physiological Sciences Graduate Interdisciplinary Program at the University of Arizona, Tucson, Arizona, January 30-31, 2003.
  • External examiner for Ph.D. candidate Nathalie Gaudreault, Department of Physiology, College of Medicine, University of British Columbia, 2004.
  • External evaluator of candidates for Associate Professor for Physiology of Microcirculation for Search Committee, Faculty of Medicine, Ludwig Maximilians Universitat, Muenchen, Germany, 2005.
  • Chair, Internal Advisory Committee, for NIH PPG, PI. Harold Laughlin and Doug Bowles, University of Missouri, Columbia, MO, 2010.
  • Invited external examiner and graduate student committee member for Calvin Chu student of Professor Vincent Moy, Department of Physiology and Biophysics, University of Miami, FL, 2010-present.

Scientific Journals

Editorships
  • Editor-in-Chief for Frontiers in Vascular Physiology, January 2010-present.
  • Co-Editor (US) for Journal of Vascular Research, August 1999-Present.
  • Consulting Editor for American Journal of Physiology: Heart and Circulatory Physiology, May 2007-January 2010.
  • Associate Editor for American Journal of Physiology: Heart and Circulatory Physiology, January 1993-January 1999.
  • Editorial Board Member for Frontiers in Cardiovascular and Smooth Muscle Pharmacology, 2010-present.
  • Editorial Board Member for International Journal of Physiology, Pathophysiology and Pharmacology, 2010-present.
  • Editorial Board Member for Nanotechnology, Science and Applications, 2007-present.
  • Editorial Board Member for Microcirculation, 1993-1999; 2003-2009; 2010-2015.
  • Editorial board member for Journal of Vascular Research, 1998-1999.
  • Editorial Board Member for Microvascular Research, 1992-1999.
  • Editorial Board Member for Hypertension, 1990-1994.
  • Editorial Board Member for American Journal of Physiology: Heart and Circ. Physiol., 1986-1993.
  • Editorial Board Member for Microcirculation, Endothelium and Lymphatics, 1988-1992.
Reviewer
  • American Journal of Physiology: (Heart and Circ. Physiology)
  • American Journal of Physiology: (Regulatory, Integ. and Comp. Physiology)
  • American Journal of Physiology: (Renal, Fluid and Elec. Physiology)
  • American Journal of Physiology: (Gastrointestinal and Liver Physiology)
  • American Journal of Physiology: (Cell Physiology)
  • Arteriosclerosis and Thrombosis and Vascular Biology
  • Biology of Reproduction
  • British Journal of Pharmacology
  • Circulation Research
  • Diabetologia
  • European Journal of Physiology
  • European Journal of Pharmacology
  • Experimental Cell Research
  • Hypertension
  • Journal of Biomechanical Engineering
  • Journal of Cell Biology
  • Journal of Fundamental and Clinical Pharmacology
  • Journal of Applied Physiology
  • Journal of Cardiovascular Research
  • Journal of Diabetes and its Complications
  • Journal of Experimental Pharmacology and Experimental Therapeutics
  • Journal of Vascular Research
  • Life Science
  • Microvascular Research
  • Nature Methods
  • Nature Nanotechnology
Selected Publications
  • Martinez-Lemus LA, X Wu, E Wilson, MA Hill, GE Davis, MJ Davis and GA Meininger. Integrins as Unique Receptors for Vascular Control.
  • J of Vasc Res 40:211-233, 2003.
  • Martinez-Lemus LA, SS Bolz, MA Hill, U Pohl and GA Meininger. Acute mechanoadaptation of vascular smooth muscle cells in response to continuous arteriolar vasoconstriction: Implications to functional remodeling. FASEB J 18(6):708-710, 2004.
  • Na S, Z Sun, GA Meininger and JD Humphrey. On atomic force microscopy and the constitutive behavior of living cells. Biomech and Modeling Mechanobiol 3:75-84, 2004.
  • Martinez-Lemus LA, Z Sun, A Trache, JP Trzeciakowski and Meininger GA. Integrins and Regulation of the Microcirculation: From Arterioles to Molecular Studies using Atomic Force Microscopy. Microcirculation, 12: 1-14, 2005.
  • Sarin, V., R. Gaffin, M.J. Davis, G.A. Meininger* and M. Muthuchamy. "RGD" containing peptides inhibit the force production of mouse papillary muscle bundles via α5β1 integrin. Journal of Physiology, 564.2:603-617, 2005. *{Shared senior authorship}
  • Martinez-Lemus, L.A., T. Crow, M.J. Davis and G.A. Meininger. Integrin dependence of the vascular myogenic response. American Journal of Physiology (Heart Circ. Physiol.), 289:H322-H329, 2005.
  • Partridge, C.R., E.S. Williams, R. Barhoumi, M. Tadesse, C.D. Johnson, K.P. Lu, G.A. Meininger, E. Wilson, K.S. Ramos. Novel genomic targets in oxidant-induced vascular injury. Journal of Molecular and Cellular Cardiology, 38:983-996, 2005.
  • Trache, A. and G.A. Meininger. An atomic force-multioptical imaging integrated microscope for monitoring molecular dynamics in live cells. Journal of Biological Optics, 2005. (In Press)
  • Trache, A., J.P. Trzeciakowski, L. Gardiner, Z. Sun, M. Muthuchamy, M. Guo, E. Wilson, S. Yuan, and G.A. Meininger. Effect of histamine on integrin a5b1 - Fibronectin interaction on endothelial cells studied using atomic force microscopy. Biophysical Journal, 89:2888-28898, 2005.
  • Sun, Z., A. Trache, L.A. Martinez-Lemus, J.P. Trzeciakowski, G.E. Davis, U. Pohl and G.A. Meininger. Regulation of Integrin-fibronectin adhesion in vascular smooth muscle cells: Studies using of atomic force microscopy. American Journal of Physiology (Heart Circ. Physiol.), 2005. (In Press)
  • Trache, A. and G.A. Meininger. An atomic force-multioptical imaging integrated microscope for monitoring molecular dynamics in live cells. Journal of Biological Optics 10(6):064023-1 – 064023-17, 2005. (This work made the cover of Biphotonics, April 2006.) PMID: 16409088
  • Chao, J.T., J.D. Neiger, G.A. Meininger and E. Wilson. Modulation of integrin-mediated adhesion and expression by Platelet-Derived Growth Factor. American Journal of Physiology (Cell Physiol.) 290:C972-980, 2006. PMID: 16282198
  • Chowdhury MH., A. Gant, A. Trache, A. Baldwin, G.A. Meininger and G.L. Cot'e. Use of surface enhanced raman spectroscopy (SERS) for the detection of human integrins. Journal of Biological Optics, 11(2):024004, 2006. PMID: 16674194
  • Gaffin, R.D., K. Gokulan, J.C. Sacchettini, T.E. Hewett, R. Klevitsky, J. Robbins, V. Sarin, D.C. Zawieja, G.A. Meininger and M. Muthuchamy. Changes in the end-to-end interactions of tropomyosin affect mouse cardiac muscle dynamics. American Journal of Physiology (Heart Circ. Physiology), 291:H552-563, 2006. PMID: 16501024
  • Hawkins, D.M., A. Trache, E.A. Ellis, D. Stevenson, A. Holzenburg, G.A. Meininger, S.M. Reddy. Quantification and confocal imaging of protein specific molecularly imprinted polymers (HydroMIPs). Biomacromolecules, 7:2560-2564, 2006. PMID: 16961318
  • Na, S., G.A. Meininger and J.D. Humphrey. A theoretical model for F-actin remodeling in vascular smooth muscle cells subjected to cyclic stretch. Journal of Theoretical Biology, 246:87-99, 2007. PMID: 17240401
  • Wu X., Y. Yang, P. Gui, Y. Sohma, G.A. Meininger, G.E. Davis, A.P. Braun and M.J. Davis. Potentiation of BK Channels by a5b1 Integrin Activation in Arteriolar Smooth Muscle. Journal of Physiology, 586.6: 1699-1713, 2008. PMID: 18218680
  • Na, S., A. Trache, Z. Sun, G.A. Meininger and J.D. Humphrey. Time-dependent changes in smooth muscle cell stiffness and focal adhesion area in response to cyclic stretch. Annals of Biomedical Engineering, 36:369-80, 2008. PMID: 18214679
  • Meissner, K.E., Z. Sun, B. Nathwani, C. Needham, R.E. Becknam, W. Everett, X. Fan, G.L. Cote and G.A. Meininger. Combining AFM and FRET for studies at the cellular level. Proceedings of the SPIE, 6863; 686302, 1-9, 2008.
  • Sun, Z., L.A. Martinez-Lemus, M.A. Hill and G.A. Meininger. Extracellular Matrix Specific Focal Adhesions in Vascular Smooth Muscle Produce Mechanically Active Adhesion Sites. American Journal of Physiology (Cell Physiology), 295:C268-C278, 2008. PMID: 18495809
  • Sun, Z., A. Juriani, G.A. Meininger, and K.E. Meissner. Probing Cell Surface Interactions Using AFM Cantilevers Functionalized for Quantum Dot-Enabled FRET. Journal of Biological Optics, 14:040502, 2009. Also selected for July 15, 2009 issue of Virtual Journal of Biological Physics Research. PMID: 19725707
  • Martinez-Lemus L.A., M.A. Hill, G.A. Meininger. The plastic nature of the vascular wall: Reply to Lee, Sandow and deMey. Letters to the Editor. Physiology, 24:273-275, 2009. PMID: 19196651
  • Huang S., Z. Sun, Z. Li and G.A. Meininger. Modulation of microvascular smooth muscle adhesion and mechanotransduction by integrin-linked kinase. Microcirculation, 17:113-127, 2010. PMID: 20163538
  • Trache, A., J.P. Trzeciakowski, G.A. Meininger. Mg2+ modulates integrin-extracellular matrix interactions in vascular smooth muscle as studied by atomic force microscopy. Journal of Molecular Recognition, 23:316-321, 2010. PMID: 20049771
  • Wu X., Z. Sun, A. Foskett, J.P. Trzeciakowski, G.A. Meininger, M. Muthuchamy. Cardiomyocyte contractile status is associated with differences in fibronection and integrin interactions. American Journal of Physiology (Heart and Circulatory Physiology), 298:H2071-H2081, 2010. {Shared senior authorship} PMID: 20382852
  • Meininger, G.A. Grand challenges in vascular physiology. Frontiers in Physiology: Frontiers in Vascular Physiology, 1:18, 2010.
  • Hongyu Qiu, Yi Zhu, Dorothy E. Vatner, Meredith Gansner, Athanasios P. Peppas, Christophe Depre, Ranillo R.G. Resuello, Filipinas F. Natividad, William C. Hunter, Guy M. Genin, Elliot L. Elson, Zhe Sun, Jerome P. Trzeciakowski, Gerald A. Meininger, Stephen F. Vatner. Vascular Smooth Muscle Stiffness as a Mechanism for Increased Aortic Stiffness with Aging. Circulation Research, 107:615-619, 2010. (Shared Senior Authorship, Co-Corresponding Authors). PMID: 20634486
  • Martinez-Lemus L.A., M.A. Hill, G.A. Meininger. The plastic nature of the vascular wall: Reply to Bjorn Folkow. Letters to the Editor. Physiology, 24:266-277, 2010.
  • Ella S.R., Y. Yang, P.S. Clifford, J. Gulia, K.A. Dora, G.A. Meininger, M.J. Davis, and M.A. Hill. Development of an Image – Based System for Measurement of Membrane Potential, Intracellular Ca2+ and contraction in Arteriolar Smooth Muscle Cells. Microcirculation, 17(8):629-640, 2010. PMID: 21044217
  • Cheng, Qi, Z. Sun, G.A. Meininger, M. Almasri. Mechanical study of micromachined polydimethylsiloxane elastic microposts. Review of Scientific Instruments, 81(10):106104-1-106104-3, 2010. PMID: 21034132
  • Jackson-Hayes, T., Z. Sun, L.A. Martinez-Lemus, M.A. Hill and G.A. Meininger. N-Cadherin and Iintegrin blockade inhibit arteriolar myogenic reactivity but not pressure-induced increases in intracellular Ca2+. Frontiers in Physiology: Frontiers in Vascular Physiology, 1:1-8, 2010. doi: 10.3389/fphys.2010.00165
  • Wu, X, S. Chakraborty, C.L. Heaps, M.J. Davis, G.A. Meininger and M. Muthuchamy. Fibronectin increases the force production on mouse papillary muscles via a5b1 integrin. Journal of Molecular and Cellular Cardiology, 50:203-213, 2011. PMID: 20937283
  • Clark, C.G., Z. Sun, G.A. Meininger and J.T. Potts. Nicotine-induced desensitization of α7-containing nicotine acetylcholine receptors on NK1 receptor expressing medullary respiratory neurons. (Submitted to Journal of Neurophysiology), 2011.
  • Sun, Z., A. Juriani, K.E. Meissner, and G.A. Meininger. Partnering atomic force microscopy with quantum dot enabled probes for FRET to study integrin-extracellular matrix interactions. Submitted to Cellular and Molecular Bioengineering, 2011.
  • Clifford, P.S., S. Ella, A. Stupica, Z. Nourian, L.A. Martinez-Lemus, K. Dora, Y. Yang, M.J. Davis, G.A. Meininger and M.A. Hill. Adventitial elastin fibers longitudinally constrain smooth muscle cells In small arteries of skeletal muscle but not from cerebral circulation. Submitted to Circulation Research, 2011. {Shared senior authorship, Co-Corresponding Authors}
  • Huang S., Z. Sun, Z. Li and G.A. Meininger. A role for zyxin in the vascular smooth response to mechanical force. (In preparation for Microcirculation), 2011.
  • Reeves, K.J., Z. Sun, G.A. Meininger and N.J. Brown. Selective measurement and manipulation of adhesion forces between cancer cells and bone marrow endothelial cells using Atomic Force Microscopy (AFM). (In preparation for Microcirculation), 2011.

 

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