Dr. Singh’s research is spans from basic to translational research. His current research includes three components.
First, HIV drug resistance. Dr. Singh is studying the mechanisms of drug resistance and susceptibility of HIV-1 reverse transcriptase (HIV-1 RT), integrase (HIV-1 IN) and protease (HIV-1 PR) in diverse HIV subtypes. He is studying the mechanism of HIV-1 resistance to nucleoside and non-nucleoside reverse transcriptase inhibitors, integrase strand transfer inhibitors, and protease inhibitors. It is understood that the efficacy of anti-HIV regimen varies among HIV subtypes. However, recent research from Dr. Singh’s lab shows that the drug susceptibility of HIV is also strain-specific. Hence, the focus of his HIV research now includes the strain-specific efficacies of anti-HIV drugs.
Second, Dr. Singh’s lab has been studying the mechanism of nucleic acid metabolism by bacterial, viral and mammalian nucleic acid polymerases and helicases. The primary goal of this component has been to elucidate the mechanism(s) of coordination among different functions of nucleic acid polymerases and helicases using advanced kinetics approaches.
The third component of Dr. Singh’s research is the discovery of drugs targeting viruses (other than HIV) and cancer. Dr. Singh has identified small molecule inhibitors targeting of Foot-and-Mouth Disease Virus (FMDV) 3Dpol (an RNA dependent RNA polymerase), SARS-CoV entry and replication inhibitors, and anticancer compounds.
401 Bond Life Sciences Center
Columbia, MO 65211
- Biochemistry, virology and molecular mechanism of drug susceptibility and resistance of HIV-1, SARS
- Mechanism of M. tuberculosis DNA replication
- Drug-discovery and development
Areas of Expertise
- Biochemistry of bacterial and viral replication
- Computer-aided drug-design
- Protein-ligand interaction