Profile
Academic Information
Office
M610G Medical Sciences Building
Columbia, MO 65212
United States
Research Interests
- Recombinant adeno-associated virus (rAAV) transduction biology
- Duchenne muscular dystrophy (DMD) gene therapy
- Duchenne cardiomyopathy
- Canine model of muscular dystrophy
- Free radical in muscle function and aging
Areas of Expertise
- Virology and Molecular Therapies
- Gene Therapy
- Neuromuscular biology
- Virology
Related Links
Education & Training
Post-Graduate School
1997, PhD, University of Pennsylvania
Awards & Honors
- 2019 - Fellow, National Academy of Inventors
- 2017 – Excellence in Research Mentoring of Trainees Award, University of Missouri, School of Medicine
- 2017 – Honorary Medical Alumni Award, University of Missouri
- 2014 – Present Member, Solid GT Scientific Advisory Committee
- 2014 – Present Member, Parent Project Muscular Dystrophy (PPMD) Scientific Advisory Committee
- 2011 – Present Editorial board “PLoS Currents: Muscular Dystrophy”
- 2011 – Book editor for “Muscle Gene Therapy: Methods and Protocols” (Humana Press, New York, NY)
- 2010 – Book editor for “Muscle Gene Therapy” (Springer, New York, NY)
- 2010 – 2016 Member, Muscular Dystrophy Association (MDA) Scientific Advisory Committee
- 2009 – Chancellor’s Award for Outstanding Research and Creative Activity, University of Missouri
- 2009 – Present Editorial board “Molecular Therapy”
- 2008 – Present Editorial board “Gene Therapy”
- 2008 – 2012 NIH SMEP Study Section charter member
- 2008 – Margaret Proctor Mulligan Professor, University of Missouri
- 2006 – Outstanding New Investigator Award, American Society of Gene Therapy
- 2004 – Spurgeon Distinguished Medical Research Award, University of Missouri
Publications
- Wasala NB, Yue Y, Lostal W, Wasala LP, Niranjan N, Hajjar RJ, Babu G, Duan D. Life-long amelioration of Duchenne dilated cardiomyopathy by SERCA2a therapy. Molecular Therapy, In-press 2019.
- Hakim CH, Lessa TB, Jenkins GJ, Yang NN, Ambrosio CE, Duan D. An improved method to study mouse diaphragm function. Scientific Report, In-press, 2019.
- Duan D. Micro-utrophin therapy for Duchenne muscular dystrophy. Molecular Therapy 27(11):1872-1874, 2019.
- Nance ME, Shi R, Hakim CH, Wasala NB, Yue Y, Pan X, Zhang T, Robinson CA, Duan SX, Yao G, Yang NN, Chen S-J, Wagner KR, Gersbach CA, and Duan D. AAV9 edits muscle stem cells in normal and dystrophic adult mice. Molecular Therapy 27(9):1568-1585, 2019
- Pan X, Sands S, Yue Y, Zheng K, LeVine SM, Duan D. An engineered galactosylceramidase construct improves AAV gene therapy for Krabbe disease in twitcher mice. Human Gene Therapy 30(9):1039-51, 2019.
- Zhao J, Yang HT, Wasala LP, Zhang K, Yue Y, Duan D*, Lai Y*. Dystrophin R16/17 protein therapy restores sarcolemmal nNOS in trans and improves muscle perfusion and function. Molecular Medicine 25(1):31, 2019 (*, co-corresponding author)
- Niranjan N, Mareedu S, Tian Y, Kodippili K, Fefelova N, Voit A, Xie L-H, Duan D, Babu GJ. Sarcolipin overexpression impairs myogenic differentiation in Duchenne muscular dystrophy. American Journal of Physiology-Cell Physiology 317(4):C813-C824, 2019.
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Zhang D, Hurst T, Duan D, Chen S-J. Unified energetics analysis unravels SpCas9 cleavage activity for optimal gRNA design. Proceedings of National Academy of Science, 116(18):8693-8698, 2019.
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Wasala NB, Hakim CH, Chen S-J, Yang NN, Duan D. Questions answered and unanswered by the first CRISPR editing study in the canine model of Duchenne muscular dystrophy. Human Gene Therapy, 30(5):535-543, 2019.
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Patel A, Zhao J, Duan D, Lai Y. Design of AAV vectors for Delivery of Large or Multiple Transgenes. Methods in Molecular Biology, 1950:19-33, 2019.
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Nance ME, Duan D. Development of next generation muscle gene therapy vectors. Muscle Gene Therapy 2nd edition (Publisher: Springer.) Duan D and Mendel JR (Ed.), 2019. p193-206.
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Duan D. Considerations on preclinical muscle gene therapy studies. Muscle Gene Therapy 2nd edition (Publisher: Springer.) Duan D and Mendel JR (Ed.), 2019. p291-326.
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Lai Y, Duan D. Design of muscle gene therapy expression cassette. Muscle Gene Therapy 2nd edition (Publisher: Springer.) Duan D and Mendel JR (Ed.), 2019. p141-156.
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Wasala LP, Hakim CH, Yue Y, Yang NN, Duan D. Systemic delivery of adeno-associated viral vectors in mice and dogs Methods in Molecular Biology 1937:281-294, 2019.
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Hakim CH, Wasala NB, Nelson CE, Wasala LP, Yue Y, Louderman JA, Lessa TB, Zhang K, Jenkins GJ, Nance ME, Pan X, Kodippili K, Yang NN, Chen S-J, Gersbach CA, Duan D. AAV CRISPR editing rescues cardiac and muscle function for 18 months. JCI Insight 3(23): e124297, 2018.
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Duan D. CRISPR alleviates muscular dystrophy in dogs. Nature Biomedical Engineering, 2(11):795-796, 2018
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Duan D. Systemic AAV micro-dystrophin gene therapy for Duchenne muscular dystrophy. Molecular Therapy 26(10):2337-2356, 2018
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Duan D. Micro-dystrophin gene therapy goes systemic in Duchenne muscular dystrophy patients. Human Gene Therapy 29(7):733-736, 2018
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Kodippili K, Hakim CH, Yang HT, Pan X, Yang NN, Laughlin MH, Terjung RL, Duan D. Nitric oxide dependent attenuation of norepinephrine-induced vasoconstriction is impaired in the canine model of Duchenne muscular dystrophy. Journal of Physiology 596(21):5199-5216
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Kodippili K, Duan D. Expressing full-length dystrophin using adeno-associated virus. Gene Therapy in Neurological Disorders Elsevier (Publisher) Ed: Mingjie Li and Joy Snider Chapter 13, 259-276, 2018
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Jenkins JG, Hakim CH, Yang NN, Yao G, Duan D. Automatic characterization of stride parameters in canines with a single wearable inertial sensor. PLoS One 13(6): e0198893, 2018.
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Wasala NB, Shin J-H, Lai Y, Yue Y, Duan D. Cardiac specific expression of ∆H2-R15 mini-dystrophin normalized all ECG abnormalities and the end-diastolic volume in a 23-m-old mouse model of Duchenne dilated cardiomyopathy. Human Gene Therapy 29(7):737-748, 2018
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Patel A, Zhao J, Yue Y, Zhang K, Duan D*, Lai Y*. Dystrophin R16/17-syntrophin PDZ fusion protein restores sarcolemmal nNOS. Skeletal Muscle 8:36, 2018. https://doi.org/10.1186/s13395-018-0182-x (*, co-corresponding author).
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Nelson DM, Lindsay A, Judge LM, Duan D, Chamberlain JS, Lowe DA, Ervasti JM. Variable rescue of microtubule and physiological phenotypes in mdx muscle expressing different miniaturized dystrophins. Human Molecular Genetics 27(12):2090-2100, 2018.
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Nance ME, Duan D. Gene therapy: use of viruses as vectors. Reference Module in Biomedical Sciences Elsevier. 10-Apr-2018 doi:10.1016/B978-0-12-801238-3.95711-8, 2018. https://doi.org/10.1016/B978-0-12-801238-3.95711-8
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Wang Y, Ravanfar M, Zhang K, Duan D, Yao G. Automatic quantification of microscopic heart damage in a mouse model of Duchenne muscular dystrophy using optical polarization tractography. Journal of Biophotonics 11(4):e201700284, 2018.
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Gordish-Dressman H, Willmann R, Dalle Pazze L, Kreibich A, van Putten M, Heydemann A, Bogdanik L, Lutz C, Davies K, Demonbruen AR, Duan D, Elsey D, Fukada SI, Girgenrath M, Patrick Gonzalez J, Grounds MD, Nichols A, Partridge T, Passini M, Sanarica F, Schnell FJ, Wells DJ, Yokota T, Young CS, Zhong Z, Spurney C, Spencer M, De Luca A, Nagaraju K, Aartsma-Rus A. "Of Mice and Measures": A Project to Improve How We Advance Duchenne Muscular Dystrophy Therapies to the Clinic (First Workshop Report: Examining current findings and opportunities around the emerging D2.B10-Dmdmdx/J (D2/mdx) model in context of the classic C57BL/10ScSn-Dmdmdx/J (Bl10/mdx)). Journal of Neuromuscular Diseases 5(4):407-417, 2018.
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Kodippili K, Pan X, Yang HT, Hakim CH, Yue Y, Zhang Y, Shin J-H, Yang NN, Duan D. Duan AAV gene therapy for Duchenne muscular dystrophy with a 7-kb mini-dystrophin gene in the canine model. Human Gene Therapy 29(3):299-311, 2018 (Journal cover image)
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Nance ME, Hakim CH, Yang NN and Duan D. Nanotherapy for Duchenne muscular dystrophy. WIREs Nanomedicine and Nanobiotechnology 18(2):e1472, 2018 (Journal cover image)