Profile
Academic Information
Margaret Proctor Mulligan Professor in Medical Research Professor
Office
M610G Medical Sciences Building
Columbia, MO 65212
United States
P. 573-884-9584
Research Interests
- Recombinant adeno-associated virus (rAAV) transduction biology
- Duchenne muscular dystrophy (DMD) gene therapy
- Duchenne cardiomyopathy
- Canine model of muscular dystrophy
- Free radical in muscle function and aging
Areas of Expertise
- Virology and Molecular Therapies
- Gene Therapy
- Neuromuscular biology
- Virology
Education & Training
Post-Graduate School
1997, PhD, University of Pennsylvania
Awards & Honors
- 2017, Honorary Medical Alumni Award, University of Missouri
- 2014 – Present Member, Solid GT Scientific Advisory Committee
- 2014 – Present Member, Parent Project Muscular Dystrophy (PPMD) Scientific Advisory Committee
- 2011 – Present Editorial board “PLoS Currents: Muscular Dystrophy”
- 2011, Book editor for “Muscle Gene Therapy: Methods and Protocols” (Humana Press, New York, NY)
- 2010, Book editor for “Muscle Gene Therapy” (Springer, New York, NY)
- 2010 – 2016 Member, Muscular Dystrophy Association (MDA) Scientific Advisory Committee
- 2009, Chancellor’s Award for Outstanding Research and Creative Activity, University of Missouri
- 2009 – Present Editorial board “Molecular Therapy”
- 2008 – Present Editorial board “Gene Therapy”
- 2008 – 2012 NIH SMEP Study Section charter member
- 2008, Margaret Proctor Mulligan Professor, University of Missouri
- 2006, Outstanding New Investigator Award, American Society of Gene Therapy
- 2004, Spurgeon Distinguished Medical Research Award, University of Missouri
Publications
- Nelson DM, Lindsay A, Judge LM, Duan D, Chamberlain JS, Lowe DA, Ervasti JM. Variable rescue of microtubule and physiological phenotypes in mdx muscle expressing different miniaturized dystrophins. Human Molecular Genetics In-press, 2018.
- Duan D. Micro-dystrophin gene therapy goes systemic in Duchenne muscular dystrophy patients. Human Gene Therapy In-press, 2018.
- Nance ME, Duan D. Gene therapy: use of viruses as vectors. Reference Module in Biomedical Sciences In-press, 2018.
- Wasala NB, Shin J-H, Lai Y, Yue Y, Duan D. Cardiac specific expression of ∆H2-R15 mini-dystrophin normalized all ECG abnormalities and the end-diastolic volume in a 23-m-old mouse model of Duchenne dilated cardiomyopathy. Human Gene Therapy In-press, 2018.
- Wang Y, Ravanfar M, Zhang K, Duan D, Yao G. Automatic quantification of microscopic heart damage in a mouse model of Duchenne muscular dystrophy using optical polarization tractography. Journal of Biophotonics In-press, 2018.
- Kodippili K, Pan X, Yang HT, Hakim CH, Yue Y, Zhang Y, Shin J-H, Yang NN, Duan D. Duan AAV gene therapy for Duchenne muscular dystrophy with a 7-kb mini-dystrophin gene in the canine model. Human Gene Therapy 29(3):299-311, 2018 (Journal cover image)
- Nance ME, Hakim CH, Yang NN and Duan D. Nanotherapy for Duchenne muscular dystrophy. WIREs Nanomedicine and Nanobiotechnology 18(2):e1472, 2018 (Journal cover image)
- Wang Y, Zhang K, Duan D, Yao G. Heart structure remodeling in the mdx4cv mouse model of Duchenne cardiomyopathy revealed using optical polarization tractography. Biomedical Optics Express 8(3):1271-1276, 2017.
- Duan D. A new kid in the playground of CRISPR DMD therapy. Human Gene Therapy Clinical Development 28(2):62-64, 2017.
- Voit A, Patel V, Pachon R, Shah V, Bakhutma M, Kohlbrenner E, McArdle J, Dell’Italia LJ, Mendell JR, Xie L-H, Hajjar RJ, Duan D, Fraidenraich D, Babu GJ. Reducing sarcolipin expression mitigates Duchenne muscular dystrophy and associated cardiomyopathy in mice. Nature Communication 8:1068, 2017.
- Hakim CH, Wasala NB, Pan X, Kodippili K, Yue Y, Zhang K, Yao G, Haffner B, Duan XS, Schneider JS, Yang NN, Chamberlain JS, Duan D. A five-repeat micro-dystrophin gene ameliorated dystrophic phenotype in the severe DBA/2J-mdx model of Duchenne muscular dystrophy. Molecular Therapy-Methods & Clinical Development In-press, 2017
- Azinfar L, Ravanfar M, Wang Y, Zhang K, Duan D, Yao G. High resolution imaging of the fibrous microstructure of the bovine carotid artery with optical polarization tractography. Journal of Biophotonics 10(2):231-241, 2017
- Xu X, Duan D, Chen S. CRISPR-Cas9 cleavage efficiency correlates strongly with target-sgRNA folding stability: from physical mechanism to off-target assessment. Scientific Reports 7:143, 2017.
- Hakim CH, Mijailovic A, Lessa TB, Coates JR, Rutkove SB, Duan D. Non-invasive evaluation of muscle disease in the canine model of Duchenne muscular dystrophy by electrical impedance myography. PLoS One, 12(3):e0173557, 2017.
- Wasala NB, Yue Y, Jenna Vance, Duan D. Uniform low-level dystrophin expression in the heart partially preserved cardiac function in an aged mouse model of Duchenne cardiomyopathy. Journal of Molecular and Cellular Cardiology 102:45-52, 2017.
- Nelson CE, Hakim CH, Ousterout DG, Thakore P, Moreb EA, Castellanos R, Madhavan S, Pan X, Asokan A, Zhang F, Duan D and Gersbach CA. Genome editing with CRISPR/Cas9 restores dystrophin expression and improves muscle function in an adult mouse model of Duchenne muscular dystrophy. Science 6271:403-407, 2016.
- Duan D, Rafael-Fortney JA, Blain A, Kass DA, McNally EM, Metzger JM, Spurney CF, Kinnett K. Standard operating procedures (SOPs) for preclinical Duchenne muscular dystrophy cardiomyopathy studies. Journal of Cardiovascular Translational Research 9(1):85-86, 2016.
- Yue Y, Binalsheikh IM, Leach SB, Domeier TL, Duan D. Prospect of gene therapy for cardiomyopathy in hereditary muscular dystrophy. Expert Opinion on Orphan Drugs 4(2):169-183, 2016.
- Duan D. Dystrophin gene replacement and gene repair therapy for Duchenne muscular dystrophy in 2016. Human Gene Therapy Clinical Development. 27(1):9-18, 2016.
- Zhao J, Kodippili K, Yue Y, Hakim CH, Wasala L, Pan X, Zhang K, Yang NN, Duan D, Lai Y. 2016. Dystrophin contains multiple independent membrane-binding domains. Human Molecular Genetics 25(10):3647-3653, 2016. (DD and YL as co-corresponding authors).
- Wasala NB, Lai Y, Shin J-H, Zhao J, Yue Y, Duan D. Genomic removal of a therapeutic mini-dystrophin gene from adult mice elicits a Duchenne muscular dystrophy-like phenotype. Human Molecular Genetics 25(13):2633-2644, 2016.
- Yue Y, Wasala NB, Bostick B, Duan D. 100-fold but not 50-fold dystrophin overexpression aggravates electrocardiographic defects in the mdx model of Duchenne muscular dystrophy. Molecular Therapy-Methods & Clinical Development. 3:16045, 2016.
- Duan D. Systemic delivery of adeno-associated viral vectors. Current Opinion in Virology 21:16-25, 2016.
- Wang Y, Ravanfar M, Zhang K, Duan D, Yao G. Mapping 3D fiber orientation in tissue using dual-angle optical polarization tractography. Biomedical Optics Express. 7(10):3855-3870, 2016.
- Yao X, Wang Y, Ravanfar M, Pfeiffer FM, Duan D, Yao G. Imaging fiber structure in cartilage using optical polarization tractography. Journal of Biomedical Optics, 21(11):116004, 2016.
- McGreevy JW, Hakim CH, McIntosh M, Duan D. Animal models for Duchenne muscular dystrophy: from basic mechanisms to gene therapy. Disease Model and Mechanism 8(3):195-213, 2015.
- Wasala NB, Zhang K, Wasala PL, Hakim CH, Duan D. FVB background does not dramatically alter dystrophic phenotype in mdx mice. PLoS Currents Muscular Dystrophy. 7. pii: ecurrents.md.28266819ca0ec5fefcac767ea9a3461c, 2015.
- Pan X, Yue Y, Zhang K, Hakim CH, Kodippili K, McDonald T, Duan D. AAV-8 is more efficient than AAV-9 in transducing neonatal dog heart. Human Gene Therapy Methods. 26(4):54-61, 2015.
- Cheever TR, Berkley D, Braun S, Brown RH, Byrne BJ, Chamberlain JS, Cwik V, Duan D, Federoff HJ, High KA, Kaspar BK, Klinger KW, Larkindale J, Lincecum J, Mavilio F, McDonald CL, McLaughlin J, McLeod BW, Mendell JR, Nuckolls G, Stedman HH, Tagle DA, Vandenberghe LH, Wilson JM, Wernett PJ, Wang H, Porter JD, Gubitz AK . Perspectives on best practices for gene therapy programs. Human Gene Therapy 26(3):127-133, 2015.
- Duan D, Hakim CH, Ambrosio C, Smith B, Sweeney L. Early loss of ambulation is not a representative clinical feature in Duchenne muscular dystrophy dogs. Disease Model and Mechanism 8(3):193-194, 2015.
- McNally EM, Kaltman JR, Benson DW, Canter CE, Cripe LH, Duan D, Finder JD, Hoffman EP, Judge DP, Kertesz N, Kinnett K, Kirsch R, Metzger JM, Pearson GD, Rafael-Fortney JA, Raman SV, Spurney CF, Targum SL, Wagner KR, Markham LW. Contemporary Cardiac Issues in Duchenne Muscular Dystrophy. Circulation 131:1590-1598, 2015.
- Wang Y, Zhang K, Wasala NB, Duan D, Yao G. Optical polarization tractography revealed significant fiber disarray in skeletal muscle of a mouse model for Duchenne muscular dystrophy. Biomedical Optics Express. 6(2):347-352, 2015.
- Duan D. Duchenne muscular dystrophy gene therapy in the canine model. Human Gene Therapy Clinical Development. 26(3):157-169, 2015.
- Yue Y, Pan X, Hakim CH, Kodippili K, Zhang K, Shin J-H, Yang HS, McDonald T, Duan D. Safe and bodywide muscle transduction in young adult Duchenne muscular dystrophy dogs with adeno-associated virus. Human Molecular Genetics. 24(20):5880-5890, 2015. (highlighted in Human Gene Therapy Clinical Development 26(4):213-214, 2015. Also reported in Fox News, Forbes and NIH Research Matters).
- Hakim CH, Peters AA, Feng F, Yao G, Duan D. Night activity reduction is a signature physiological biomarker for Duchenne muscular dystrophy dogs. Journal of Neuromuscular Diseases. 2(4):397-407, 2015.
- Nance ME and Duan D. Perspective on adeno-associated virus (AAV) capsid modification for Duchenne muscular dystrophy gene therapy. Human Gene Therapy 16(12):786-800, 2015.
- Lai Y, Zhao J, Yue Y, Wasala NB, Duan D. Partial restoration of cardiac function with ∆PDZ nNOS in aged mdx model of Duchenne cardiomyopathy. Human Molecular Genetics, 23(12):3189-3199, 2014. PMCID:PMC4030774.
- Kodippili K, Vince L, Shin J-H, Yue Y, Mooris G, McIntosh MA, Duan D. Characterization of 65 epitope-specific dystrophin monoclonal antibodies in canine and murine models of Duchenne muscular dystrophy by immunostaining and western blot. PLoS One, 9(2):e88280, 2014.
- Hakim CH, Yue Y, Shin J-H, Williams RR, Zhang K, Smith BF, Duan D. Systemic gene transfer reveals distinctive muscle transduction profile of tyrosine mutant AAV-1, -6 and -9 in neonatal dogs. Molecular Therapy-Methods & Clinical Development. 1:14002, 2014.
- Lostal W, Kodippili K, Yue Y, Duan D. Full-length dystrophin reconstitution with adeno-associated viral vectors. Human Gene Therapy 25(6):552-62, 2014.
- Wang Y, Zhang K, Wasala NB, Yao X, Duan D, Yao G. Histology validation of mapping depth-resolved cardiac fiber orientation in fresh mouse heart using optical polarization tractography. Biomedical Optics Express 5(8):2843-55, 2014. PMCID: PMC4133011
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