Have you ever come across a stagnant pool that smelled like rotten eggs when you disturbed it? Or, have you wondered why iron pipes corrode in soil? Both the smell and the corrosion are caused by the sulfate-reducing bacteria that derive energy not from oxygen, but from sulfate, which is reduced to hydrogen sulfide. Do these bacteria only cause problems? Probably not, because we believe they can assist bioremediation, the destruction of toxic contaminants in the environment. Since oxygen kills these bacteria, all work with them must be carried out in the absence of air. Obviously, none but the most committed (stubborn) would work with them. We are investigating the genetics and metabolism of hydrogen, iron, and sulfate in these bacteria. How do they corrode iron? Metallic iron can serve as reductant and the Fe2+ produced is water soluble, until it is irrevocably precipitated by the sulfide ions also produced by the bacteria. Are the bacteria caught in a vicious cycle of removing iron that they need for growth, by the sulfide that is produced during growth? Do they need an iron acquisition system to grow? The laboratory is also looking at the potential application of these bacteria to bioremediation of heavy metal contaminated environments. In particular their ability to change the solubility of toxic metals that may make the metals less biologically available. Our studies abound with Southerns, cloning, PCRs, transposons, and problems. What we need now is a little intelligent help in understanding these fascinating and challenging organisms.
214 Schweitzer Hall
Columbia, MO 65211
- Bacterial Genetics
- Bacterial Pathogenesis
- Bioinformatics/ Systems Biology
- Gene Expression
Areas of Expertise
- Genetics of uranium reduction by sulfate-reducing bacteria
Education & Training
1974, PhD, Duke University
- Kurczy, Michael; Forsberg, Erica; Thorgersen, Michael; Poole, Farris; Benton, H. Paul; Ivanisevic, Julijana; Tran, Minerva; Wall, Judy; Elias, Dwayne; Adams, Michael; Siuzdak, Gary. 2016. Global isotope metabolomics reveals adaptive strategies for nitrogen assimilation in Pseudomonas. ACS Chemical Biology 11(6):1677-85. DOI: 10.1021/acschembio.6b00082. PMID: 27045776
- Shatsky M., S. Allen, B.L. Gold, N.L. Liu, T.R. Juba, S.A. Reveco, D.A. Elias, R. Prathapam, J. He, W. Yang, E.D. Szakal, H. Liu, M.E. Singer, J.T. Geller, B.R. Lam, A. Saini, V.V. Trotter, S.C. Hall, S.J. Fisher, S.E. Brenner, S.R. Chhabra, T.C. Hazen, J.D. Wall, H.E. Witkowska, M.D. Biggin, J.M. Chandonia, G. Butland. 2016. Bacterial interactomes: interacting protein partners share similar function and are validated in independent assays more frequently than previously reported. Mol Cell Proteomics. 15(5):1539-55. doi: 10.1074/mcp.M115.054692. PMID: 26873250
- Shen Q., J.D. Wall, Z. Hu. 2016. Solids retention time dependent phototrophic growth and population changes in chemostat cultivation using wastewater. Water Environ Res. 88(1):5-12. doi:10.2175/106143014X13975035526103. PMID: 26803021
- Martins M., F.O. Mourato C, Morais-Silva, C. Rodrigues-Pousada, G. Voordouw, J.D. Wall, I.A. Pereira. 2016. Electron transfer pathways of formate-driven H2 production in Desulfovibrio. Appl Microbiol Biotechnol. 100(18):8135-46. doi: 10.1007/s00253-016-7649-7. PMID: 27270746
- Christensen G.A., A.M. Wymore, A.J. King, M. Podar, R.A. Hurt Jr, E.U. Santillan, A. Soren, C.C. Brandt, S.D. Brown, A.V. Palumbo, J.D. Wall, C.C. Gilmour, D.A. Elias. 2016. Development and validation of broad-range qualitative and cade-specific quantitative molecular probes for assessing mercury methylation in the environment. Appl Environ Microbiol. 82(19):6068-78. doi: 10.1128/AEM.01271-16. PMID: 27422835
- Rajeev, L., Chen, A., Kazakov, A.E., Luning, E.G., Zane, G.M., Novichkov, P.S., Wall, J.D., and Mukhopadhyay, A. 2015. Regulation of nitrite stress response in Desulfovibrio vulgaris Hildenborough, a model sulfate-reducing bacterium. J. Bacteriol. JB.00319-15. PMID: 26283774.
- Rabus, R., Venceslau, S.S., Wöhlbrand, L., Voordouw, G., Wall, J.D., and Pereira, I.A.C. 2015. A post-genomic view of the ecophysiology, catabolism and biotechnological relevance of sulphate-reducing prokaryotes. R.K. Poole (Series Ed.) Advances in Microbial Physiology, Academic Press an imprint of Elsevier, London, Oxford, and San Diego. Adv Microb Physiol. 66:55-321. PMID: 26210106
- Zhou, A., K.L. Hillesland, Z. He, W. Schackwitz, Q. Tu, G.M. Zane, Q. Ma, Y. Qu, D.A. Stahl, J.D. Wall, T.C. Hazen, M.W. Fields, A.P. Arkin, and J. Zhou. 2015. Rapid selective sweep of pre-existing polymorphisms and slow fixation of new mutations in experimental evolution of Desulfovibrio vulgaris. ISME J. 7 April 2015 PMID: 25848870
- Smith, S.D., R. Bridou, A. Johs, J.M. Parks, D.A. Elias, R.A. Hurt Jr, S.D. Brown, M. Podar, and J.D. Wall. 2015. Site-directed mutagenesis of HgcA and HgcB reveals amino acid residues important for mercury methylation. Appl Environ Microbiol. 81(9):3205-17. doi: 10.1128/AEM.00217-15.
- Brileya, K.A., L.B. Camilleri, G.M. Zane, J.D. Wall, and M.W. Fields. 2015. Biofilm growth mode promotes maximum carrying capacity and community stability during product inhibition syntrophy. Front Microbiol. 5:693. doi: 10.3389/fmicb.2014.00693.
- Pellerin, A., L. Anderson-Trocmé, L.G. Whyte, G.M. Zane, J.D. Wall, and B.A. Wing. 2015. Sulfur isotope fractionation during the evolutionary adaptation of a sulfate-reducing bacterium. Appl Environ Microbiol. 81(8):2676-89. doi: 10.1128/AEM.03476-14.
- Korte, H.L., A. Saini, V.V. Trotter, G.P. Butland, A.P. Arkin, and J.D. Wall. 2015. Independence of nitrate and nitrite inhibition of Desulfovibrio vulgaris Hildenborough and use of nitrite as a substrate for growth. Environ Sci Technol Jan 9 [Epub ahead of print] PMID:25534748.
- Christensen, G.A., G.M. Zane, A.E. Kazakov, X. Li, D.A. Rodionov, P.S. Novichkov, I. Dubchak, A.P. Arkin, and J.D. Wall. 2015. Rex (encoded by DVU0916) in Desulfovibrio vulgaris Hildenborough is a repressor of sulfate adenylyl transferase and Is regulated by NADH. J Bacteriol. 197:29-39. doi: 10.1128/JB.02083-14
- Ramos, A.R., F. Grein, G.P. Oliveira, S.S. Venceslau, K.L. Keller, J.D. Wall, and I.A. Pereira. 2014. The FlxABCD-HdrABC proteins correspond to a novel NADH dehydrogenase/heterodisulfide reductase widespread in anaerobic bacteria and involved in ethanol metabolism in Desulfovibrio vulgaris Hildenborough. Environ Microbiol. doi: 10.1111/1462-2920.12689 [Epub ahead of print]
- Rajeev, L., E.G. Luning, S. Altenburg, G.M. Zane, E.E. Baidoo, M. Catena, J.D. Keasling, J.D. Wall, M.W. Fields, and A. Mukhopadhyay. 2014. Identification of a cyclic-di-GMP-modulating response regulator that impacts biofilm formation in a model sulfate reducing bacterium. Front Microbiol. 5:382. doi: 10.3389/fmicb.2014.00382.
- Hillesland, K.L., S. Lim, J.J. Flowers, S. Turkarslan, N. Pinel, G.M. Zane, N. Elliott, Y. Qin, L. Wu, N.S. Baliga, J. Zhou, J.D. Wall, and D.A. Stahl. 2014. Erosion of functional independence early in the evolution of a microbial mutualism. Proc Natl Acad Sci USA. 111(41):14822-7. doi: 10.1073/pnas.1407986111.
- Ray, J., K.L. Keller, M. Catena, T.R. Juba, M. Zemla, L. Rajeev, B. Knierim, G.M. Zane, J.J. Robertson, M. Auer, J.D. Wall, and A. Mukhopadhyay. 2014. Exploring the role of CheA3 in Desulfovibrio vulgaris Hildenborough motility. Front Microbiol. 5:77. doi: 10.3389/fmicb.2014.00077.
- Keller, K.L., B.J. Rapp-Giles, E.S. Semkiw, I. Porat, S.D. Brown, and J.D. Wall. 2014. A new model for electron flow for sulfate reduction in Desulfovibrio alaskensis G20. Appl Environ Microbiol. PMID:24242254
- Korte, H.L., S.R. Fels, G.A. Christensen, M.N. Price, J.V. Kuehl, G.M. Zane, A.M. Deutschbauer, A.P. Arkin, and J.D. Wall.2014. Genetic basis for nitrate resistance in Desulfovibrio strains. Front Microbiol. 5:153 doi: 10.3389/fmicb.2014.00153. PMID:24795702
- Fels, S.R., G.M. Zane, S.M. Blake, and J.D. Wall. 2013. Rapid transposon liquid enrichment sequencing (TnLE-seq) for gene fitness evaluation in underdeveloped bacterial systems. Appl Environ Microbiol 79(23):7510-7. doi: 10.1128/AEM.02051-13. PMID: 24077707